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. 2019 Sep 3;11:8167–8179. doi: 10.2147/CMAR.S209904

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© 2019 Yang et al.

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HOXC6 regulates the proliferation and clonogenicity of GBM cells. (A) The effect of HOXC6 knockdown with shRNA and shNC was verified by Western blotting 48 hrs after transfection. (B) The effect of HOXC6 overexpression was verified by Western blotting. (C) Viability of HOXC6 knockdown cells was assessed with a CCK-8 assay at indicated times. (D) Viability of HOXC6 overexpression cells was assessed with a CCK-8 assay at indicated times. (E) Colony formation assays of U251 and U87 cells infected with shNC or HOXC6-targeted shRNAs and the relative statistical analysis are shown by column diagram. (F) Colony formation assays of HOXC6 overexpression cell lines. All experiments were carried out in triplicate. Data are presented as mean ± standard deviation. Statistically significant difference: *p<0.05, **p<0.01, ***p<0.001.