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. 2019 Sep 3;14:7123–7139. doi: 10.2147/IJN.S211682

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© 2019 Czarnomysy et al.

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Flow cytometric analysis of keratinocyte and fibroblast cancer cells after incubation with 2nd and 3rd generation PAMAM dendrimers (0.3 mg/mL, 1.5 mg/mL and 3.0 mg/mL) for 24 h and subsequent staining with Annexin V and propidium iodide (PI). Dots with Annexin V−/PI− (left lower square), Annexin V+/PI− (right bottom square), Annexin V+/PI+ (right upper square), and Annexin V−/PI+ (left upper square) feature represent intact, early apoptotic, late apoptotic, and necrotic cells, respectively. Mean percentage values from three independent experiments (n=3) done in duplicate are presented. *p<0.05 versus the control group.