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. 2019 Sep 3;12:7203–7214. doi: 10.2147/OTT.S194800

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© 2019 Liang et al.

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MiR-765 targets APE1 in OS cells. (A) Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis in five osteosarcoma cancer cell lines showed an inverse expression trend between miR-765 and APE1 expression. The expression levels of miR-765 and APE1 were normalized to hFOB1.19. (B) The predicted duplex formation between human WT APE1 3ʹ-UTR and miR-765 is shown. (C) The WT andMut APE1 3ʹ-UTR sequences are shown. (D) Schematic diagram showing luciferase reporter constructs containing the APE1 3ʹ-UTR sequences. (E) Effects of miR-765 mimics (inhibitors) on APE1 3ʹ-UTR luciferase reporters in HOS cells. Empty vector pmirGLO-Report and negative control mimics (N) were included as controls. Luciferase activities were calculated as the ratio of ﬁreﬂy/renilla activities and normalized to the negative control mimics group. The results were obtained from three independent experiments, with each experiment conducted in triplicate. Data are shown as the mean ± SD. (F and G) Western blot analysis was performed to quantify the intracellular APE1 levels. Actin was used as an internal control.