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. Author manuscript; available in PMC: 2020 Aug 22.
Published in final edited form as: Cell. 2019 Aug 8;178(5):1132–1144.e10. doi: 10.1016/j.cell.2019.07.001

Figure 2. Selfish centromeres enrich more MT-destabilizing factors through the BUB1 pathway.

Figure 2.

(A and C) CF-1 x CHPO (L x S) hybrid oocytes, or CF-1 x CF-1 (L x L) as controls, were fixed at metaphase I and stained for phosphorylated INCENP, Survivin, MCAK, BUB1, H2ApT121, or SGO2. Graph shows centromere signal ratios, calculated as the brighter divided by the dimmer signal for each bivalent (n > 32 for each condition); red line, mean; *P < 0.001. (B) CF-1 x CHPO hybrid oocytes expressing CENP-B-EGFP were stained for pINCENP or MCAK. Graph shows centromere signal ratios, calculated as the CF-1 centromere divided by the CHPO centromere signal for each bivalent. Each dot represents a single bivalent (n > 31 for each condition); red line, mean; *P < 0.001, indicating significant deviation from 1. Images (A-C) are maximum intensity z-projections showing all chromosomes (left), or optical slices magnified to show single bivalents (right); scale bars, 10 μm. (D) Model of the amplified BUB1 pathway in larger centromeres compared to smaller centromeres in the intraspecific CHPO hybrid.