Fig. 5.

a Flow cytometric analysis of cellular internalization of the nanoparticles labelled with FRET dyes by MIA PaCa-2 cells show a time dependent uptake (Black: control, Blue: dye labelled nanoparticles at 24 h, Red: at 48 h and Green at 72 h) (b) Detection of FRET associated fluorescence (540 nm) in the perinuclear space of MIA PaCa-2 cancer cells, when the cells were incubated with nanoparticles conjugated with FRET coupled dyes, i.e. AF 594 and AF 647. Arrow indicates the presence of nanoparticles around the nucleus which were stained with DAPI (c) Western blot data showing the cellular availability of SCH 772984, suppressing the phosphorylation of ERK (A: Encapsulated ERK inhibitor (1 nM), B: Free ERK inhibitor (1 nM), C: Bare Nanoparticles, D: Encapsulated ERK inhibitor (varying concentrations), E: Free ERK inhibitor (varying concentrations), F: Bare NCs (varying concentrations)