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. 2019 Sep 8;8(9):giz105. doi: 10.1093/gigascience/giz105

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© The Author(s) 2019. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4: — rCASC graphical interface within 4seqGUI. A, Count table generation menu: this set of functions is devoted to the conversion of fastq to a count table. B, Count table manipulation menu: this set of functions provides inspection, filtering, and normalization of the count table. C, Clustering menu: these functions allow the use of SIMLR, t-SNE, Seurat, griph, and scanpy to group cells in subpopulations. D, Feature selection menu: this set of functions allows the identification of cluster-specific subsets of genes and their visualization using heat maps.