LOX-1 Ab treatment attenuates MVE-exposure induced BBB permeability. Changes in BBB permeability were assessed using the fluorescent tracer, sodium fluorescein (Na-F). ApoE−/− mice were exposed by inhalation to either mixed vehicular engine exhaust (MVE: 100μg PM/m3)+ LOX-1 Ab (16mg/ml, 0.1 ml/mouse, every other day throughout 30 d exposure); MVE + IgG (16 mg/ ml, 0.1 ml/mouse); filtered air (FA) + LOX-1 Ab; or FA + IgG, and a subset of mice (n = 6 per group) were injected i.p. with 100 μl of 2% Na-F-PBS 30min prior to the end of their final exposure on day 30. Graph shows total fluorescence in the cerebrum, as measured by fluorometry. Data are expressed as the amount of tracer per gram of tissue. *p < 0.050 compared to FA + IgG control; †p < 0.050 compared to MVE + IgG, as determined by ANOVA.