Fig. 2.

Representative hematoxylin and eosin (H&E)-stained sections of motor cortex in pigs that underwent sham surgery and normothermic recovery (a), sustained hypothermia (b), or hypothermia with rewarming (c). Shown are low magnification panoramic and higher resolution (insets) views to appreciate the general anatomic locations and the cellular details. a In a sham normothermic piglet, neurons in layers 2 and 3 appear morphologically viable. b, c After hypothermia and hypothermia with rewarming, subsets of neurons are characterized by vacuolated, eosinophilic cytoplasm and nuclei that are dark and condensed but maintain nucleoli (arrows). The neuropil also shows spongiform changes. Photos taken at 200× magnification and insets taken at 600× magnification. Scale bar is 50 μm.