Fig. 6.

Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining to identify cell death in anterior putamen (a–c) and posterior putamen (d–f). a Temperature and HI interacted in their effect on the number of TUNEL+ cells (p = 0.035). * p < 0.05. b Interrater reliability analysis showed significant correlation in counting TUNEL+ cells and apoptotic profiles by hematoxylin and eosin (H&E) at the anterior level (r = 0.65, p < 0.0001). c Bland-Altman plot for TUNEL+ cells and apoptotic cells by H&E stain at the anterior level. Bias is shown by the solid line. The 95% limits of agreement are shown by dotted lines. Bias was near-zero with high agreement. d Temperature independently affected the number of TUNEL+ cells (p < 0.001), and HI and cooling had an interactive effect (p < 0.001) ** p < 0.01. e Interrater reliability analysis showed significant correlation in counting TUNEL+ cells and apoptotic profiles by H&E at the posterior level (r = 0.57, p < 0.0001). f Bland-Altman plot for TUNEL+ cells and apoptotic cells by H&E stain at the posterior level. Bias is shown by the solid line. The 95% limits of agreement are shown by dotted lines. Bias was near-zero with high agreement. Each circle represents 1 piglet. Data in (a, b) are graphed as box plots with IQRs and 5–95th percentile whiskers. Analyses in (a, b) were conducted by 2-way analysis of variance and post hoc pairwise comparisons with Holm-Sidak tests. NormoT, sustained normothermia; HypoT, sustained hypothermia; Rewarm, hypothermia followed by rewarming.