Table 1.
Primary antibodies used for Western blotting
| Antibody | Dilution used | Manufacturer | Catalog number | Clonality | Role |
|---|---|---|---|---|---|
| Mouse anti-neuronal nuclei IgG | 1:5,000 | Millipore; Temecula, CA, USA | MAB377 | Monoclonal | Specific neuronal marker |
| Goat anti-cleaved caspase 3 IgG | 1:1,000 | R&D Systems; Minneapolis, MD, USA | AF-605-NA | Polyclonal | Mediator of apoptosis |
| Rabbit anti-TNF-α IgG | 1:100 | Abcam; Cambridge, UK | Ab6671 | Polyclonal | Marker of inflammation |
| Mouse anti-CD95 IgG | 1:100 | BD Transduction Laboratories; San Jose, CA, USA | 610197 | Monoclonal | Mediator of apoptosis |
| Rabbit anti-nitrotyrosine IgG | 1:25,000 | Millipore; Darmstadt, Germany | 06–284 | Polyclonal | Marker of inflammation |
| Rabbit anti-Iba1 IgG | 1:1,000 | Wako; Richmond, VA, USA | 016–20001 | Polyclonal | Microglia/macrophage marker |
| Mouse anti-HSP70 IgG | 1:1,000 | Abcam; Cambridge, UK | Ab2787 | Monoclonal | Involved in unfolded protein response |
| Mouse anti-ERN1 IgG | 1:500 | Abgent; San Diego, CA, USA | AO1361a | Monoclonal | Involved in unfolded protein response |
| Rabbit anti-PERK IgG | 1:1,000 | Cell Signaling Technology; Danvers, MA, USA | 3192 | Monoclonal | Involved in unfolded protein response |
| Rabbit anti-pERN1 IgG | 1:1,000 | Novus Biologicals; Littleton, CO, USA | NB100–2323 | Polyclonal | Involved in unfolded protein response |