Figure 2.
Hypovolemic dehydration impaired performance in the novel object recognition task in a sex/hormone dependent manner. (A) Rats treated with furosemide consumed significantly more water and 1.5% saline compared to rats treated with saline control, which confirmed that the treatment induced dehydration. (B) General activity was influenced by group and treatment. E and saline-treated D2 rats were more active than male and furosemide-treated D2 rats. (C) Regardless of treatment, both groups of males and E rats spent significantly more time investigating the novel, compared to the original object. In D2 rats, however, only the control-treated group spent more time investigating the novel object. Abbreviations: males (M), diestrous 2 females (D2), estrous females (E), saline control (C), furosemide (F). Hashed bars denote the treatment groups. +Greater than control-treated, p < 0.001. aGreater than control and furosemide-treated males and furosemide-treated D2 females, p < 0.05. bGreater than control males and furosemide-treated D2 females, p < 0.05. cGreater than control males, p < 0.01. *Greater than time investigating original object, p < 0.001.