Figure 3.

HOXA13 is a miR‐128‐3p direct target. (a) Prediction of binding site between HOXA13 and miR‐128‐3p and construction of HOXA13‐MUT plasmids. (b) Detection of the luciferase activity in NT2 and 293T after co‐transfection plasmid (pGL3‐HOXA13‐WT or pGL3‐HOXA13‐MUT) and miRNA mimics examined via dual‐luciferase reporter gene experiments. (c) The mRNA level of HOXA13 in Hypo patients is remarkably decreased. (d) The protein expression level of HOXA13 in Hypo patients is obviously decreased. Data are presented as mean ± SD. *p ≤ .05, Student’s t‐test