Figure 4.

HOTTIP/miR‐128‐3p regulatory loop is pivotal for HOXA13 expression. (a) NT2 is transfected with miR‐128‐3p inhibitors (with or without HOTTIP siRNAs) and the mRNA level of HOXA13 is assessed via qRT‐PCR. (b) Following NT2 is treated with miR‐128‐3p inhibitors (with or without HOTTIP siRNAs), western blotting is carried out to detect the HOXA13 protein level, with GAPDH as a control. (c) 293T is transfected with miR‐128‐3p mimics (with or without HOTTIP OE plasmids), and the relative mRNA level of HOXA13 is detected using qRT‐PCR. (d) 293T is transfected with miR‐128‐3p mimics (with or without HOTTIP OE plasmids), and the relative protein level of HOXA13 is measured through qRT‐PCR. Data are presented as mean ± SD. *p ≤ .05, Student’s t‐test