Figure 3.

PPAR‐γ is a potential miR‐130 target. (a) The 3′‐UTR sequence of PPAR‐γ was screened and aligned to identify complementarity between PPAR‐γ and both miR‐130a and miR‐130b. (b) The suspected binding of miR‐130 with the wild‐type 3′‐UTR region of PPAR‐γ mRNA is shown. A mutated 3′‐UTR of PPAR‐γ is also shown. (c) Dual‐luciferase reporter gene assay showed the direct functional interactions between PPAR‐γ and miR‐130. Data were shown as mean ± SD, firefly luciferase activity was normalized to Renilla luciferase activity, ***p < .001 compared to wild‐type 3′‐UTR of PPAR‐γ cotransfected with control miRNA