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. 2019 Jul 5;27(9):1558–1567. doi: 10.1016/j.ymthe.2019.06.013

Figure 5.

Figure 5

LGR5+ Cells as Cancer Stem Cell Identification

(A) β-catenin mRNA expression in LGR5+ and LGR5− cells were detected by qPCR after FACS sorting. *p < 0.05. (B) H&E staining of the vehicle and treated tumor samples. The arrow indicates LGR5+ cell patches. (C) TUNEL assay of vehicle and treated tumor samples. Blue indicates nuclear staining, and the arrow indicates DNA fragments caused by apoptosis. (D) Western blot of LGR5 in vehicle and treatment groups (N = 4). (E) FACS analysis of LGR5+ cells in the vehicle group and treatment group.