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. 2019 Sep 6;39(9):BSR20191523. doi: 10.1042/BSR20191523

Figure 4. miR-16-5p overexpression reversed SNHG1-inhibited aberrant catabolism and inflammation triggered by IL-1β stimulation.

Figure 4

Normal human articular chondrocytes-keen were transfected with lnc-SNHG1, lnc-SNHG1+ miR-NC, or lnc-SNHG1+ miR-16-5p, respectively and then treated with IL-1β (10 ng/ml) for 24 h. (A) Western blot was performed to detect the protein expression levels of MMP-1, MMP-3, MMP-9, ADAMTS-4, ADAMTS-5, aggrecan, collagen II, i-NOS, and COX-2. (B,C,D,F) Quantitation of band intensity in (A). (E) ELISA was performed to measure the levels of IL-6 and TNF-α. ‘*’ means compared with control group P<0.05, and ‘#’ means compared with lnc-SNHG1or lnc-SNHG1+ miR-NC group P<0.05. GAPHD was used as an invariant internal control for calculating protein-fold changes.