. 2019 Sep 6;39(9):BSR20191874. doi: 10.1042/BSR20191874

Table 1. Inhibition constants (K_i,app), Hill coefficients (h) and apparent dissociation constants derived from DSF experiments (K_D,app) for wild-type NQO1 and the variants p.R139W and p.P187S.

Inhibitor	Wild-type NQO1	p.R139W	p.P187S
	K_i,app
Dicoumarol	1.15 ± 0.51 nM	2.98 ± 1.04 nM	10.95 ± 1.11 nM²
Nicotinamide	14.01 ± 4.34 mM	13.55 ± 3.22 mM	nd
Resveratrol	375 ±129 μM	nd	nd
	Hill coefficient (h)
Dicoumarol	0.56 ± 0.09	0.51 ± 0.09	Inactive
Dicoumarol + excess FAD	0.47 ± 0.09¹,²	0.60 ± 0.04²	0.48 ± 0.02²
Resveratrol	0.89 ± 0.08	0.85 ± 0.12	nd
Nicotinamide	0.91 ± 0.06	0.92 ± 0.05	nd
	K_D,app
Dicoumarol	54 ± 4 nM	66 ± 11 nM	1100 ± 280 nM
Nicotinamide	6.0 ± 0.9 mM	18.2 ± 5.2 mM	nd

h is the mean value from three separate linear Hill plots ± the standard deviation of this mean.

Previously reported and included here for comparison [39].

In excess FAD (10×[active site]).

nd, not determined.

Table 1. Inhibition constants (Ki,app), Hill coefficients (h) and apparent dissociation constants derived from DSF experiments (KD,app) for wild-type NQO1 and the variants p.R139W and p.P187S.