Fig. 7.

Intensity of TREK-2, TRAAK, and TRESK channel staining in Müller glia cultures following pressure elevation. Primary, purified cultures of Müller glia were exposed to ambient (Amb) or elevated (Elev) pressure for 48 h. A: representative fluorescent micrographs of Müller glia from each condition. Immunolabeling of TREK-2 (green), TRAAK (green), TRESK (green), and CD44 (red) was performed. Panels directly below the fluoromicrographs are a z-plane view of the image, with associated plot of fluorescent intensity. B: bar graphs of the mean intensity of TREK-2, TRAAK, and TRESK staining compared between ambient and elevated pressure. TREK-2: n(Amb) = 24 cells; n(Elev) =21; TRAAK, n(Amb) = 19; n(Elev) = 21; TRESK: n(Amb) = 12; n(Elev) = 22. Images taken at ×60; scale bar = 40 µm. Inset taken at ×60 + ×2.5 zoom; scale bar = 10 µm. Student’s t-test was used to analyze statistical significance. *P < 0.05, **P < 0.005.