Fig. 11.

Inhibition of cation channel activity in Müller glia by fluoxetine (Fluox) treatment. Primary, purified cultures of Müller glia were exposed to ambient (Amb) or elevated (Elev) pressure for 4 h, in the presence of vehicle or 100 µM fluoxetine. A: representative heat map showing the fluorescent signal of Thallos dye in Müller glia from each condition. Images were taken after the addition of thallium. B: line graphs displaying the normalized fluorescence intensity of Thallos dyes over time for each condition. Student’s t-test was used to analyze statistical significance. C: box plots of the average fluorescence intensity following the addition of thallium compared between each condition. One-way ANOVA with pairwise comparison by Dunn’s method was used to analyze statistical significance. n(Amb) = 5 cells; n(Elev) = 12; n(Amb+Fluox) = 28; n(Elev+Fluox) = 22. *P < 0.05.