Fig. 3.

Chronic hypoxia (CH) does not significantly alter acid-sensing ion channel (ASIC)2 or ASIC3 protein levels in intrapulmonary arteries. A: representative Western blots of ASIC2 (predicted ∼69 kDa), ASIC3 (predicted ∼59 kDa), and corresponding Coomassie-stained blot. All samples were collected at the same time and processed in parallel on 2 gels for each antibody to accommodate 12 samples [6 control (Con) and 6 CH] from male wild-type (WT) mice. B: summary data detecting ASIC2/3 expression (normalized to entire lane on Coomassie-stained blot) in isolated intrapulmonary arteries from Con and CH WT mice. Values are means ± SE; dots indicate n = 6/group, analyzed by unpaired t-test.