Fig. 2.
PIEZO1-tandem-dimer Tomato (tdT) expression and distribution in the mouse kidney. A−E: expression of PIEZO1-tdT in the cortex (A), cortex/outer stripe of the outer medulla (B), inner stripe of the outer medulla (C), inner medulla (D), or renal pelvis (E) of Piezo1tdT/tdT or Piezo1+/+ mice. Tissue was labeled with antibodies that detect PIEZO1-tdT, FITC-phalloidin to label the F-actin cytoskeleton, and TO-PRO-3 to label nuclei. *Renal corpuscles in A. In B, the yellow dashed lines indicate the interface between the cortex (top of image) and outer stripe of the outer medulla (bottom of image). In E, the boxed region of the urothelium lining the renal pelvis is magnified in the inset. Arrows point to the location of the junctional complex, which sits at border between the basolateral and apical plasma membrane domains. The lumen is to the right. The strong F-actin staining (green) is associated with smooth muscle cells that underlie the urothelium.