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. 2019 Jun 5;317(2):F303–F321. doi: 10.1152/ajprenal.00214.2019

Fig. 5.

Fig. 5.

PIEZO1-tandem-dimer Tomato (tdT) expression in the collecting ducts (CDs) of the mouse kidney. A–E: localization of PIEZO1-tdT, aquaporin-2 (AQP2), V-ATPase subunit E1 (ATP6V1E1), and pendrin (SLC26A4) in the kidney. In A, the insets show that PIEZO1-tdT was localized to the basolateral surfaces of AQP2-positive principal cells in the cortical CDs (labeled with yellow circles). B: PIEZO1-tdT colocalized with E-cadherin (CDH1) at the basolateral surfaces of CDs. The inset in C shows that PIEZO1-tdT was associated with the basolateral surfaces of an ATP6V1E1-positive type A intercalated cell, which is distinct from AQP2-positive principal cells. The inset in D shows a cell where PIEZO1-tdT was localized to the basolateral surface of type B interstitial cells expressing apical SLC26A4. The inset in E shows that PIEZO1-tdT was localized to the basolateral surfaces of the cells lining the medullary CD.