Fig. 9.
PIEZO1-tandem-dimer Tomato (tdT) expression in the prostate, seminal vesicles, and ejaculatory ducts of male mice. A–F: distribution of PIEZO1-tdT, actin, and nuclei (Nuc) in the dorsal prostate (PT; A), lateral PT (B), anterior PT (C), ventral PT (D), seminal vesicles (E), and ejaculatory ducts (terminal region of ductus deferens; F) of Piezo1tdT/tdT and Piezo1+/+ mice. The actin signal associated with each organ is saturated to show the relatively weak signal associated with the epithelium of these organs. In the dorsal PT, lateral PT, and anterior PT, PIEZO1-tdT was localized primarily to stromal cells surrounding the epithelium. In the ventral PT, PIEZO1-tdT was localized to the stromal cells as well as the basolateral surface of the cuboidal epithelium that lines this gland of the ventral PT. The red circle in B, right, indicates nonspecific staining observed in glands containing large amounts of prostatic secretions. Arrows in E indicate the stromal regions within the papilla that form the seminal vesicles.