Table 3.

Transmission electron microscopy ultrastructural analysis of the two-stage uIRI model

Parameter	Two-Stage uIRI-0 Min Group	Two-Stage uIRI-15 Min Group	Two-Stage uIRI-18 Min Group	Two-Stage uIRI-21 Min Group	Two-Stage uIRI-24 Min Group
Peritubular interstitium	Normal	Normal	Normal	Wide	Wide
Collagen	−	−	−	+	+
Cellular debris in Bowman’s space	−	−	−	+	+
Glomerular basement membrane thickness, nm	131 ± 37	164 ± 52	148 ± 33	286 ± 61*	362 ± 89*
Foot process width, nm	136 ± 44	132 ± 35	182 ± 52	624 ± 78*	962 ± 113*

Peritubular interstitium expansion, collagen deposition, cellular debris within Bowman’s space, glomerular basement membrane thickening, and podocyte foot process effacement were detected in the two-stage unilateral ischemia-reperfusion injury (uIRI)-21 min and two-stage uIRI-24 min groups but not in two-stage uIRI-15 min, two-stage uIRI-18 min, or two-stage uIRI-0 min control groups (n = 5 fields/mice, 11–13 mice/group).

^*P < 0.01 vs. the two-stage uIRI-0 min group.