Obeticholic acid (OCA) ameliorates weight gain and white adipose tissue inflammation in nonpregnant high-fat diet (HFD)-fed mice. A: weight gain in nonpregnant (D0) and pregnant (D18) mice before and after HFD prefeeding and at gestational days (GD) 7, 14, and 18. P < 0.05 as determined by two-way ANOVA followed by Tukey’s multiple comparisons test for the following comparisons: b, normal chow (NC) vs. HFD; c, HFD vs. HFD OCA. B: body weight (BW) at GD18, without uterine horn. C: liver weight at GD18 normalized to BW without uterine horn. D: relative mRNA expression of inflammatory markers in gonadal white adipose tissue at GD18. *P < 0.05 vs. NC control, #P < 0.05 vs. HFD control as determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are expressed as means ± SE; n = 5–11 mice per group.