Effect of obeticholic acid (OCA) on expression of bile acid homeostasis genes is altered by pregnancy. Relative mRNA expression of targets in farnesoid X receptor signaling pathways in maternal liver and distal ileum. A: hepatic gene expression in pregnant mice (D18) relative to nonpregnant mice (D0). Data were analyzed by unpaired Student’s t-test; n = 5–6 mice per group. B: hepatic gene expression in D0 and D18 mice. *P < 0.05 vs. normal chow (NC), **P < 0.01 vs. NC, #P < 0.05 vs. high-fat diet (HFD), as determined by one-way ANOVA followed by Tukey’s multiple comparisons test; n = 5–6 mice per group. C: distal ileal gene expression in D18 relative to D0 mice. Data were analyzed by unpaired Student’s t-test; n = 5–6 mice per group. D: distal ileal gene expression in D0 and D18 mice. *P < 0.05 vs. NC, **P < 0.01 vs. NC, #P < 0.05 vs. HFD, as determined by one-way ANOVA followed by Tukey’s multiple comparisons test; n = 5–6 mice per group.