Fig. 5.

Cyclophilin D (CypD) expression in mouse kidney mitochondrial extract and in mouse glomerular endothelial cells (MGECs). A: wild-type (WT) and Akita mice were supplemented with or without GYY4137 (GYY) for 8 wk, and kidney cortical tissue was analyzed by Western blotting. Bar diagram represents densitometric CypD analysis of immunoblots. B: MGECs were incubated without or with GYY and MK-801 (a specific NMDA-R blocker) in NG or HG conditions for 24 h. Isolated mitochondrial lysate was immunoblotted against CypD antibody. Bar diagram represents densitometric CypD analysis. Values are mean ± SE, n = 5 or 6/group; *,†<0.05 and ϕP > 0.05