Fig. 5.
T regulatory cell depletion increases hemodynamic response to angiotensin II (ANG II) infusion in 129S6 premenopausal female mice. Administration of anti-CD25 to ANG II-infused (800 ng·kg−1·min−1) premenopausal 129S6 mice (anti-CD25/ANG) significantly reduced the frequency of splenic (A) and renal (B) T regulatory cells in ANG II-infused mice compared with ANG II and control mice (n = 4/group). Representative flow cytometric gating images are shown for each corresponding group and tissue. C: premenopausal female mice administered anti-CD25 exhibited a significantly greater increase in systolic blood pressure (SBP) after 14 days of ANG II infusion. Results are expressed as means ± SE; n = 10 mice/group. *P < 0.05 vs. control; #P < 0.05 vs. ANG II, unpaired Student’s t-test.