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. 2019 Jun 7;317(2):H445–H459. doi: 10.1152/ajpheart.00738.2018

Fig. 3.

Fig. 3.

Validation of relative expression of genes obtained from RNA-seq by quantitative PCR. Relative quantification normalized to 18s. [n = 5 per group; saline vs. electronic cigarette (e-cigarette) (2.4%), *P < 0.05, **P < 0.01; e-cigarette (0%) vs. e-cigarette (2.4%), #P < 0.05, ##P < 0.01]. COL1a1, collagen type I α1; COL3a1, collagen type III α1; Col5a3, collagen type V α3; Hrk, harakiri; Npas2, neuronal PAS domain protein 2; Per2, period circadian protein homolog 2; Per3, period circadian protein homolog 3; Ddit4m, DNA damage inducible transcript 4 like; SCD1, stearoyl-CoA desaturase-1; Timp4, tissue inhibitor of metalloproteinase 4; TNF RSM 12A, Tumor necrosis factor receptor superfamily member 12A; Wisp2, WNT1 inducible signaling pathway protein 2.