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Activity of free Asp (black squares), Asp entrapped in L3 bulk phase (dark gray solid circles), and in L3-NPs (light gray triangles) as a function of time. Note that day 0 is 1 week after the free enzyme solution and bulk phase samples were prepared because bulk phases require some equilibration time. L3 NPs were prepared from a bulk phase a day before the first measurement. All samples were stored at room temperature.
