Table 4.
Peak Position, i.e. Elution Volume, and AUC Obtained for the Lipid NPs and Free Asp Peaks as a Result of SEC for L3-NPs Without Enzyme and Encapsulated Asp Analysed During Three Consecutive Days
| Sample | L3-NPs |
Aspartic Protease |
||
|---|---|---|---|---|
| Peak Maximum (mL) | AUC (mAU · mL) | Peak Maximum (mL) | AUC (mAU · mL) | |
| 1.5 mg/mL free Asp | – | – | 18.38 | 17.88 |
| 40 mg/mL unloaded L3-NPs | 8.03 | 603.63 | – | – |
| Encapsulated Asp - day 1 | 8.09 | 808.39 | 18.35 | 3.14 |
| Encapsulated Asp - day 2 | 8.03 | 1063.06 | 18.21 | 3.95 |
| Encapsulated Asp - day 3 | 7.90 | 989.70 | 18.40 | 3.21 |
| Encapsulated Asp - 5 months old | 7.74 | 827.86 | – | – |