Table 1.
Sequence of primers used for qRT-PCR.
| Genes | Sequence ID | Forward primers (5′-3′) | Reverse primers (5′-3′) | Product size (bp) |
|---|---|---|---|---|
| HPRT1 | NM_000194.3 | CAAAGATGGTCAAGGTCGCA | TCAAATCCAACAAAGTCTGGCT | 82 |
| CXCL8 | NM_000584.4 | TCAGAGACAGCAGAGCACAC | ACACAGTGAGATGGTTCCTTCC | 76 |
| IL6 | NM_000600.5 | AACCTGAACCTTCCAAAGATGG | TCTGGCTTGTTCCTCACTACT | 159 |
| IL1B | XM_017003988.2 | GGACAGGATATGGAGCAACAAGTGG | TTCAACACGCAGGACAGGTACAGAT | 125 |
| IFNB1 | NM_002176.4 | GTCACTGTGCCTGGACCATA | AATTGTCCAGTCCCAGAGGC | 122 |
| TNF | NM_000594.4 | TCAGCAAGGACAGCAGAGGA | GTCAGTATGTGAGAGGAAGAGAACC | 128 |
| TLR3 | NM_003265.2 | GCTCTGGAAACACGCAAACC | CTCGTCAAAGCCGTTGGACT | 97 |
| TLR4 | NM_138554.5 | TGGTGTGTCGGTCCTCAGTGT | GCAGCCAGCAAGAAGCATCAG | 91 |
| TLR7 | NM_016562.4 | TCTTGCCTTCTGGAGTTTTTG | CAGTGGTCAGTTGGTTGTGG | 150 |
| IFIH1 | NM_022168.4 | GGAGTCAAAGCCCACCATCT | AGACCTTCTTCTGCCACTGTG | 146 |
| DDX58 | NM_014314.4 | GCCTTGGCATGTTACACAGC | TTTGGCTTGGGATGTGGTCT | 110 |
TLR: toll-like receptor; IFIH1: interferon-induced helicase C domain-containing protein 1, known as MDA5; DDX58: DExD/H-box helicase 58 (known as RIG-I).