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. 2019 Sep 3;12:7215–7226. doi: 10.2147/OTT.S211535

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© 2019 Sun et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Silencing of miR-21 suppresses the proliferation, migration and invasion of pancreatic cancer cells. (A) PANC-1 and MiaPaca-2 cells were infected with lenti-AS-21 or lenti-NC. Reverse transcription-quantitative polymerase chain reaction analysis was used to confirm the knockdown of miR-21 in PANC-1 and MiaPaca-2 cells after 24 h infection. (B) An MTT assay was used to detect the viability of PANC-1 cells infected with lenti-AS-21 or lenti-NC for 24, 72 or 120 h. (C) A colony formation assay was used to detect the colony formation rate of PANC-1 cells infected with lenti-AS-21 or lenti-NC. The colony formation rate was calculated using the following equation: Colony formation rate = (number of colonies/number of plated cells) x100%. (D) Transwell migration and (E) invasion assays were performed with PANC-1 cells infected with lenti-AS-21 or lenti-NC. Values are expressed as the mean ± standard deviation from three independent experiments.

Notes: *P<0.05; **P<0.01; ***P<0.001. The image color is RGB.

Abbreviations: miR, microRNA; lenti-NC, negative control lentivirus; lenti-AS-21, microRNA-21 inhibitor vector; PANC-1, human pancreatic cancer cell; Mia Paca-2, human pancreatic cancer cell.