Skip to main content
. 2019 May 31;4(9):1312–1322. doi: 10.1016/j.ekir.2019.05.1157

Figure 1.

Figure 1

Nucleoporin (NUP)93 expression in the human kidney cortex. Lysates of HEK293T cells transiently expressing FLAG-NUP93 or FLAG-laminin β2 (LAMB2) were immunoprecipitated with anti-FLAG antibody and analyzed by Western blot with the indicated antibodies (a). These immunoprecipitates were used for antibody absorption. Human kidney sections were stained with anti-NUP93 antibody preabsorbed with anti-FLAG immunoprecipitates from lysates of HEK293T cells that expressed control (flag-LAMB2) vector or flag-tagged NUP93 (b–e). The NUP93 signals were detected in cells both inside and outside of glomeruli of control kidneys with the preincubated lysate of the control vector (b,c). No staining was detected with anti-NUP93 antibody preabsorbed immunoprecipitates from lysates of flag-tagged NUP93 vector (d,e). Glomeruli are indicated by arrows. Bar = 50 μm.