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Expression of nucleoporin (NUP)93 in the patient with NUP93 mutations. Dual-labeling immunofluorescence of NUP93 (green) with CD2-associated protein (CD2AP) (red; a) and NUP93 (green) with nephrin (red; b). The NUP93 signal was decreased in the whole biopsy specimen. The expression of CD2AP was maintained (a) and that of nephrin was only partially decreased (b) in the patient’s glomeruli. Bar = 50 μm. DAPI, 4ʹ,6-diamidino-2-phenylindole.
