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. Author manuscript; available in PMC: 2019 Sep 9.
Published in final edited form as: J Mol Cell Cardiol. 2018 Jul 5;121:107–123. doi: 10.1016/j.yjmcc.2018.07.003

Fig. 4.

Fig. 4.

TWEAK induces the expression of multiple inflammatory mediators in cardiac fibroblasts in part via TRAF3IP2. Silencing TRAF3IP2 by lentiviral shRNA (moi0.5 for 48 h) inhibited TWEAK (10 ng/ml for 2 h)-induced cytokine, chemokine, growth factor and adhesion molecule mRNA expression (RT-qPCR, A). Further, silencing TRAF3IP2 reduced TWEAK-induced cytokine and chemokine secretion (ELISA, B) and protein expression of adhesion molecules (Immunoblotting, C). C, A representative image from three independent immunoblot experiments with similar results is shown at the top with summarized semi-quantification of intensity of immunoreactive bands by densitometry from all three experiments in the respective bottom panels. A, B, n = 6, C, n = 3. A-C, *P < at least 0.01 versus untreated, †P < 0.05 versus TWEAK, §P < 0.05 versus Untreated.