Fig 2.

Cellular localisation of cardiac μ-opioid receptors in the ventricular myocardium. (a) The localisation of cardiac μ-opioid receptors in normal and failing rat myocardium was examined by immunohistochemistry (n=4). Red arrows indicate positive staining (brown). Bar=50 μm. Double immunofluorescence method was used to detect the co-localisation of cardiac μ-opioid receptors (red) with (b) α-sarcomeric actin (α-SA, green) or (c) transient receptor potential vanilloid type 1 (TRPV1, green) (n=4). The nuclei stained bright blue with 4′-6-diamidino-2-phenylindole (DAPI). White arrows indicate the separate positive staining and the red arrows indicates merge staining (yellow). Bar=20 μm.