Figure 3.

hKLK1 could inhibit oxidative stress in CC of rats with HHcy. (a) Representative western blot results of p22^phox, p47^phox and gp91^phox in CC of rats. Expressions of p22^phox, p47^phox and gp91^phox with β-actin as the loading control in all four groups were presented through bar graphs: (b) for p22^phox, (c) for p47^phox and (d) for gp91^phox. (e) MDA levels determined by the ELISA method in all four groups. (f) SOD activities determined by the ELISA method in all four groups. Data are expressed as mean ± standard deviation (n = 5 per group). ^*P < 0.05, ^**P <0.01 and ^***P < 0.001. Met: methionine; hKLK1: human tissue kallikrein-1; CC: corpus cavernosum; HHcy: hyperhomocysteinemia; TGR: transgenic rats; ELISA: enzyme linked immunosorbent assay; SOD: superoxide dismutase.