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. 2019 Jan 1;21(5):508–515. doi: 10.4103/aja.aja_111_18

Supplementary Table 1.

Primers used in conventional polymerase chain reaction and real-time reverse transcriptase-polymerase chain reaction

Genes Primer sequences Usage (PCR)
hKLK1 F: 5’GTCCAGAAGGTGACAGACTTCAT3’ Conventional
R: 5’GTCCTCGATCCACTTCACATAAG3’
F: 5’CTCACAGCTGCTCATTGCATC3’
R: 5’GCTCTCACTGACATGAACAAACTGG3’
Realtime
rKLK1 F: 5’CCCACACACAGATGGTGACAGA3’ Realtime
R: 5’CCTTGAAGCACACCATCACAGAG3’
βactin F: 5’AAGAGCTATGAGCTGCCTGAI3’ Conventional and realtime
R: 5’TACGGATGTCAACGTCACAC3’

RT-PCR: reverse transcriptase-polymerase chain reaction; hKLK1: human tissue kallikrein-1; rKLK1: rat tissue kallikrein-1