Supplementary Table 1.
Genes | Primer sequences | Usage (PCR) |
---|---|---|
hKLK1 | F: 5’GTCCAGAAGGTGACAGACTTCAT3’ | Conventional |
R: 5’GTCCTCGATCCACTTCACATAAG3’ F: 5’CTCACAGCTGCTCATTGCATC3’ R: 5’GCTCTCACTGACATGAACAAACTGG3’ |
Realtime | |
rKLK1 | F: 5’CCCACACACAGATGGTGACAGA3’ | Realtime |
R: 5’CCTTGAAGCACACCATCACAGAG3’ | ||
βactin | F: 5’AAGAGCTATGAGCTGCCTGAI3’ | Conventional and realtime |
R: 5’TACGGATGTCAACGTCACAC3’ |
RT-PCR: reverse transcriptase-polymerase chain reaction; hKLK1: human tissue kallikrein-1; rKLK1: rat tissue kallikrein-1