Figure 3.

Knockdown of lncRNA ENST00000500843 increased chemoresistance to PTX in A549 cells. (A) After 36 h of transfection, relative expression levels of ENST00000500843 in A549 cells were measured by reverse transcription-quantitative PCR. Transfection of siRNA-135, siRNA-197 and siRNA-7 targeting ENST00000500843 significantly downregulated lncRNA ENST00000500843 expression in A549 cells, and siRNA-7 exhibited the most marked transfection efficiency. (B) Following transfection of siRNA-7, A549 cells were exposed to 10 ng/ml PTX for 24 h and cell viability was assessed by a Cell Counting kit-8 assay. Downregulation of ENST00000500843 increased resistance to PTX. **P<0.01 vs. si-NC; lncRNA, long non-coding RNA; NC, negative control; PTX, paclitaxel; siRNA, small interfering RNA.