Figure 2.

SAHA induces apoptosis in HepG2 cells in a dose-dependent manner. (A) Representative flow cytometry plots showing the population of apoptotic HepG2 cells treated with the specified doses of SAHA for 48 h. Cells were stained with annexin V-FITC and PI to assess apoptosis by flow cytometry. Data shown represent one of three experiments. The lower right quadrant represents the early apoptotic cells, and the upper right quadrant represents the late apoptotic and dead cells. (B) Quantification of the apoptotic rates (percentages of Annexin V-positive cells) in HepG2 cells exposed to SAHA at the indicated doses for 48 h. N=3 for each group. *P<0.05, **P<0.01. SAHA, suberoylanilide hydroxamic acid; FITC, fluorescein isothiocyanate; PI, propidium iodide; Q, quadrant.