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. Author manuscript; available in PMC: 2020 May 20.
Published in final edited form as: Dev Cell. 2019 Apr 4;49(4):542–555.e9. doi: 10.1016/j.devcel.2019.03.003

Figure 3. lep-5 lncRNA expression is temporally regulated.

Figure 3.

(A) Temporal expression of Plep- 5::GFP::NLS in late L1 animals (top) through adults (bottom). Expression is observed in the tail epidermis including the tail tip cells, in pharynx muscles, in neurons in the head and cloacal region, and in seam cells (arrowheads). (B) Temporal expression of lep-5 lncRNA from L1 to adult as measured by qPCR. Error bars show SD. (C, D) lep-5 lncRNA visualized with smFISH probes in the tail tip of a wild-type and lep-5(ny10) L2 animal. lin-44 probes were used as positive control. DAPI staining indicates nuclei. (E-G) Late L2 male. lep-5 lncRNA visualized with smFISH probes (left) and nuclei stained with DAPI (right) showing areas of concentrated lep-5 lncRNA expression in the ganglia in the pharynx (E) and rectal (F) region and in the somatic portion of the developing gonad (G). See also Figure S6.