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. Author manuscript; available in PMC: 2020 May 20.
Published in final edited form as: Dev Cell. 2019 Apr 4;49(4):542–555.e9. doi: 10.1016/j.devcel.2019.03.003

Figure 4. lep-5 functions upstream of lin-28 in the heterochronic pathway to control TTM.

Figure 4.

(A) Expression of Pdmd-3::YFP in wild-type and mutant male tail tips at early (e), mid (m) and late (l) L4 stage. (B) Penetrance and expressivity of tail tip phenotypes in single mutants, double mutants and mutant/RNAi knockdown combinations. Left column shows results in a lep-5 mutant background (relevant allele indicated); right column shows corresponding result in a wild-type background. PD = post dauer, HT115 = RNAi control bacterial strain, asterisk: RNAi fed to L1 larvae. (C) DIC images of representative L4 and adult male tails from the experiments summarized in (B). A rounded tail tip in L4 results from precocious TTM in L3. Adults shown for lin-14(RNAi) have just molted and remain surrounded by the L4 cuticle. See also Figure S7.