Table 5.
Conjugation efficiencies and the equivalent concentration of the CPPs tagged to PLGA nanoparticles (NPs) using different concentrations of CPPs prepared with a post-microfluidics conjugation approach. Data are means ± SD (n = 3 independent batches). *p-value <0.05 in comparison to the conjugation efficiency of 8.6 mM TAT.
| CPP concentration (mM) | Conjugation efficiency (%) ± SD | CPP concentration (µM) ± SD |
|---|---|---|
| RRH-tagged PLGA NPs | ||
| 10 | 32.4 ± 0.8 | 0.25 ± 0.03 |
| 50 | 74.4 ± 4.5 | 3.10 ± 0.19 |
| 75 | 58.5 ± 6.0 | 3.66 ± 0.37 |
| TAT-tagged PLGA NPs | ||
| 2.9 | 57.6 ± 0.5 | 0.150 ± 0.001 |
| 5.7 | 56.3 ± 1.0 | 0.29 ± 0.01 |
| 8.6 | 55.5 ± 0.8 | 0.43 ± 0.01 |
| bTAT-tagged PLGA NPs | ||
| 4.5 | 79.8 ± 0.3* | 0.327 ± 0.001 |
| 6.0 | 45.4 ± 0.7 | 0.248 ± 0.004 |