Fig. 4.

Effect of total lipids and bortezomib concentration on remote loading. Liposomes containing mannitol + meglumine + acetic acid (200, 50, and 30 mM, respectively) in 20 mM HEPES were prepared at 100 mM total lipids concentration and further diluted to get 25 and 50 mM total lipids. Bortezomib was added at increasing concentrations followed incubation at room temperature for remote loading. Encapsulated amount of bortezomib was measured by UPLC (A). Encapsulation efficiency was calculated (B). Data is presented as mean ± SD of two independent experiments.