Table 2.

Colon-region specific colonization of microbial community. Average abundance (%) of microbial families belonging to specific phyla in the luminal (L) and mucosal (M) environment of the ascending (AC), transverse (TC) and descending colon (DC) of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) at the end of the control period (n = 1/donor). For optimal observation of consistent effects over the different donors tested, the average of the three donors is presented (n = 3). Statistical differences between colon regions (AC vs TC vs DC) are indicated by assigning different letters to the respective colon regions (small letters are used for the luminal environment, while capital letters are used for the mucosal environment). Statistical differences between the lumen and the mucus layer are indicated by their respective p-values in each of the colon regions. P-values < 0.05 are considered as statistically significant differences.

Phylum	Family	Abundance (%)						p-value
		L			M			L vs M
		AC	TC	DC	AC	TC	DC	AC	TC	DC
Actinobacteria	Bifidobacteriaceae	7.8 a	3.0 a	3.0 a	27.6 A	16.6B	10.3B	0.006	0.006	0.024
	Coriobacteriaceae	0.0 a	0.1b	0.1b	0.0 A	0.6B	1.0B	0.374	0.079	0.016
Bacteroidetes	Bacteroidaceae	28.5 a	37.6 a	26.9 a	13.4 A	10.8 A	12.8 A	0.284	0.008	0.096
	Prevotellaceae	9.2 a	0.7 a	0.2 a	1.5 A,B	0.1 A	0.0B	0.381	0.029	0.009
	Rikenellaceae	0.0 a	0.1b	0.2b	0.0 A	0.4B	1.1C	–	0.022	0.003
	Tannerellaceae	0.5 a	6.2b	7.4b	0.2 A	1.4B	1.4B	0.532	0.049	0.040
Firmicutes	Acidaminococcaceae	0.1 a	0.8b	1.2 a,b	0.0 A	0.5B	1.4B	0.460	0.364	0.748
	Christensenellaceae	0.0 a	0.0 a	0.0 a	0.0 A	0.0 A	0.2B	–	0.145	0.012
	Clostridiaceae_1	0.0 a	0.0 a	0.0 a	0.0 A	0.1 A	0.6B	0.586	0.138	0.005
	Erysipelotrichaceae	0.0 a	0.0 a	0.1 a	0.0 A	0.1 A,B	0.2B	–	0.364	0.050
	Eubacteriaceae	0.0 a	0.0 a	0.1 a	0.0 A	0.1 A,B	1.0B	–	0.355	0.062
	Lachnospiraceae	11.0 a	30.4b	29.6b	25.6 A	24.5 A	28.9 A	0.117	0.217	0.889
	Lactobacillaceae	0.0 a	0.0 a	0.0 a	2.2 A	0.1B	0.0C	0.012	0.020	0.654
	Ruminococcaceae	0.0 a	2.2 a	2.2 a	0.0 A	1.0B	4.6C	0.463	0.543	0.161
	Veillonellaceae	31.9 a	11.4b	11.6b	19.0 A	5.3B	3.4B	0.069	0.094	0.019
Proteobacteria	Burkholderiaceae	0.2 a,b	0.2 a	0.4b	0.2 A	0.3 A	1.0 A	0.903	0.182	0.143
	Desulfovibrionaceae	0.0 a	0.8b	1.5c	0.1 A	2.9B	0.8C	0.251	0.004	0.035
	Enterobacteriaceae	10.3 a	0.5b	0.3b	9.9 A	0.3B	0.1B	0.924	0.222	0.009
	Pseudomonadaceae	0.7 a	0.3 a,b	0.2b	0.1 A	0.0B	0.0B	0.003	0.085	0.057
	uncultured	0.0 a	0.4 a	0.5 a	0.0 A	0.4 A	0.3 A	–	0.947	0.766
Synergistetes	Synergistaceae	0.0 a	2.8b	12.1c	0.0 A	34.5B	29.7B	0.214	0.007	0.015
Verrucomicrobia	Akkermansiaceae	0.0 a	2.5 a	2.0 a	0.0 A	0.1 A	0.7 A	–	0.345	0.313