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Effects of simvastatin on early adipogenic genes in 3T3-L1 preadipocytes. 3T3-L1 preadipocytes stimulated with differentiation cocktail (DC) and cigarette smoke extract (CSE) for 30–120 min in the presence or absence of simvastatin. Gene expression was quantified with RT-PCR. Cyr61 (A), Ptgs2 (B), Egr1 (C) and Zfp36 (D) with β-actin as endogenous control. Values are the mean ± s.d. of three independent experiments. *P < 0.05.
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