Figure 4.

Effects of simvastatin on the morphology in differentiated 3T3-L1 preadipocytes. Mature 3T3-L1 adipocytes stained with Oil Red O after differentiation for 6 days; no treatment (A); treatment with cigarette smoke extract (CSE) (B); treatment with differentiation cocktail (DC) (C); or treatment with simvastatin and DC (D). Cells were visualized by light microscope at ×10 magnification. Oil Red O was quantified from simvastatin-treated and untreated preadipocytes after extraction at 490 nm (E). Values are the mean ± s.d. of three independent experiments. *P < 0.05.