Figure 7.

Effects of simvastatin on adipogenesis in human primary orbital fibroblasts. Orbital fibroblasts were treated with simvastatin and differentiation cocktail (DC) for 1–12 days. Gene expression was quantified with RT-PCR of late adipogenic genes SCD (A) and PPAR-γ (B) with cyclophilin A as endogenous control. Mature adipocytes at differentiation day 12 were stained with Oil Red O and visualized by light microscope at ×20. No treatment (C) treatment with DC (D) treatment with simvastatin and DC (E). Values are the mean ± s.d. of three independent experiments. *P < 0.05.