Table 8. ELISA results against RT-PCR and IB detection.
| ELISA Alternative test |
UGV1 IgY* (n) (95%CI) |
UGV1 IgM* GM (n) (95%CI) |
UHV1 NP IgY* (n) (95%CI) |
UHV1 NP IgM* (n) (95%CI) |
UHV-1 NP-C IgY* (n) (95%CI) |
UHV-1 NP-C IgM* (n) (95%CI) |
|---|---|---|---|---|---|---|
| BIBD +ve |
0.155 (33) (0.095–0.252) |
0.561 (33) (0.479–0.657) |
0.156 (34) (0.114–0.213) |
0.250 (34) (0.202–0.308) |
0.251 (34) (0.191–0.329) |
0.290 (34) (0.241–0.351) |
| BIBD -ve |
0.553 (36) (0.337–0.906) |
0.448 (36) (0.399–0.503) |
0.306 (36) (0.244–0.385) |
0.255 (36) (0.199–0.327) |
0.556 (36) (0.452–0.682) |
0.379 (36) (0.339–0.422) |
| t-test | t = 3.7246, df = 67 P<0.001 |
t = -2.3586, df = 67 p<0.05 |
t = 3.5899, df = 68 p<0.001 |
t = 0.1294, df = 68 p = 0.903 |
t = 4.771, df = 68 p<0.001 |
t = 2.5368, df = 68 p<0.05 |
| UGV-2 RT-PCR +ve |
0.209 (42) (0.133–0.329) |
0.464 (42) (0.398–0.540) |
0.173 (43) (0.132–0.226) |
0.254 (43) (0.214–0.301) |
0.282 (43) (0.221–0.361) |
0.296 (43) (0.254–0.344) |
| UGV-2 PR-PCR -ve |
0.530 (27) (0.288–0.975) |
0.464 (27) (0.458–0.596) |
0.326 (27) (0.251–0.123) |
0.249 (27) (0.179–0.346) |
0.598 (27) (0.480–0.744) |
0.402 (27) (0.356–0.454) |
| t-test | t = 2.5322, df = 67 p<0.05 |
t = -1.181, df = 67 p = 0.2118 |
t = 3.2325, df = 68 p<0.01 |
t = -0.1199, df = 68 p = 0.9049 |
t = 4.2732, df = 68 p<0.001 |
t = 2.9145, df = 68 p<0.01 |
| S5-like RT-PCR +ve |
0.262 (60) (0.180–0.382) |
0.495 (60) (0.445–0.551) |
0.207 (61) (0.168–0.256) |
0.251 (61) (0.210–0.301) |
0.353 (61) (0.292–0.427) |
0.333 (61) (0.295–0.375) |
| S5-like PT-PCR -ve |
0.745 (9) (0.171–0.324) |
0.522 (9) (0.378–0.718) |
0.339 (9) (0.156–0.733) |
0.260 (9) (0.197–0.344) |
0.592 (9) (0.263–1.330) |
0.335 (9) (0.295–0.375) |
| t-test | t = 1.9239, df = 67 p = 0.0586 |
t = 0.3602, df = 67 p = 0.7199 |
t = 1.6353, df = 68 p = 0.1068 |
t = 0.1522, df = 68 p = 0.8795 |
t = 1.8398, df = 68 p = 0.0702 |
t = 0.041, df = 68 p = 0.9674 |
| SMTV-2 RT-PCR +ve |
0.334 (53) (0.220–0.508) |
0.506 (53) (0.452–0.567) |
0.235 (54) (0.192–0.289) |
0.259 (54) (0.213–0.315) |
0.407 (54) (0.334–0.495) |
0.359 (54) (0.320–0.402) |
| SMTV-2 PT-PCR -ve |
0.212 (16) (0.088–0.507) |
0.475 (16) (0.383–0.589) |
0.178 (16) (0.098–0.546) |
0.230 (16) (0.179–0.296) |
0.292 (16) (0.171–0.500) |
0.258 (16) (0.198–0.337) |
| t-test | t = -1.0373, df = 67 p = 0.3033 |
t = -0.5349, df = 67 p = 0.5945 |
t = -1.1503, df = 68 p = 0.2541 |
t = -0.6136, df = 68 p = 0.5415 |
t = -1.4677, df = 68 p = 0.1468 |
t = -2.6477, df = 68 p<0.05 |
| Total | 0.301 (69) (0.207–0.436) |
0.499 (69) (0.452–0.550) |
0.221 (70) (0.180–0.270) |
0.252 (70) (0.215–0.296) |
0.377 (70) (0.312–0.456) |
0.333 (70) (0.299–0.371) |
*Optical density geometric mean